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专利名称：Modified naphthalene dioxygenases and
methods of use
发明人：Rebecca Parales,David Gibson,Sol
Resnick,Kyoung Lee
申请号：US09843250申请日：20010426公开号：US06794167B2公开日：20040921
摘要：The invention provides an NDO or NDO related complex comprising at leastone alpha-subunit polypeptide that comprises: 1) a substituted amino acid at the positioncorresponding to position 352 in NDO, 2) a substituted amino acid at the positioncorresponding to position 201, 202, 260, 316, 351, 358, 362, or 366 in NDO, or 3) asubstituted amino acid at the position corresponding to position 352 in NDO, and asubstituted amino acid at the position corresponding to position 201, 202, 260, 316, 351,358, 362, or 366 in NDO; or a catalytically active fragment thereof. The invention alsoprovides DNA encoding such polypeptides, host cells augmented by such DNA, andmethods for using the enzymes or host cells to provide useful and novel synthons. Theinvention also provides novel compounds prepared with the complexes or methods ofthe invention.
申请人：UNIVERSITY OF IOWA RESEARCH FOUNDATION
代理机构：Schwegman, Lundberg, Woessner & Kluth, P.A.
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